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Product Name
CTHRC1 antibody
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Description
CTHRC1 Rabbit Polyclonal antibody. Positive WB detected in A375 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, rat lung tissue. Positive IP detected in A375 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CTHRC1 antibody; Protein NMTC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CTHRC1 recombinant protein (Accession Number: NM_138455). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:109724(CTHRC1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109724(CTHRC1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109724(CTHRC1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-CTHRC1 (IP:Catalog No:109724, 3ug; Detection:Catalog No:109724 1:500) with A375 cells lysate 3600ug.
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Background
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
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References
- Zhou S, Gao R, Hu W. MiR-9 inhibits Schwann cell migration by targeting Cthrc1 following sciatic nerve injury. Journal of cell science. 127(Pt 5):967-76. 2014.
- Yan L, Yu J, Tan F. SP1-mediated microRNA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1. American journal of cancer research. 5(4):1447-59. 2015.
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