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Product Name
CTCFL Polyclonal Antibody
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Description
Polyclonal antibody to CTCFL
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CTCFL antibody; BORIS antibody; CT27 antibody; CTCF-T antibody; HMGB1L1 antibody; dJ579F20.2 antibody; CCCTC-binding factor like antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:100 -
Validations
Western blot - CTCFL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CTCFL antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunofluorescence - CTCFL Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using CTCFL antibody . Blue: DAPI for nuclear staining.
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Background
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
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