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Product Name
CST8 Polyclonal Antibody
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Description
Polyclonal antibody to CST8
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CST8 antibody; CRES antibody; CTES5 antibody; cystatin-8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 53-142 of human CST8 (NP_005483.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CST8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CST8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - CST8 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CST8 antibody . Blue: DAPI for nuclear staining.
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Background
Performs a specialized role during sperm development and maturation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"