-
Product Name
CSNK1E Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CSNK1E
-
Tested applications
WB, IHC, IF, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
CSNK1E antibody; CKIepsilon antibody; HCKIE antibody; casein kinase 1 epsilon antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - CSNK1E Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CSNK1E antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - CSNK1E Polyclonal Antibody
Western blot analysis of extracts from normal (control) and CSNK1E knockout HeLa cells, using CSNK1E antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - CSNK1E Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - CSNK1E Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody at dilution of 1:200 (40x lens).
Immunofluorescence - CSNK1E Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CSNK1E antibody . Blue: DAPI for nuclear staining.
-
Background
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"