Crustacean Cardioactive Peptide, CCAP

Crustacean Cardioactive Peptide, CCAP

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

PFCNAFTGC-NH2 (disulfide bond: Cys3-Cys9)

H-Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2

9

98.1%

C₄₂H₅₇N₁₁O₁₁S₂

956.11

Synthetic

Powder

Native and synthetic CCAP displayed high ino- and chronotropic effects on a semi-isolated crab heart preparation. Cardioactive peptide was found in common shore crab / green crab (Carcinus maenas), tobacco hornworm / tobacco hawkmoth (Manduca sexta), yellow mealworm beetle (Tenebrio molitor), Southern armyworm (Spodoptera eridania), desert locust (Schistocerca gregaria), African malaria mosquito.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• J.Stangier et al., Proc. Natl. Acad. Sci. USA, 84, 575 (1986)
• H.K.Lehman et al., Peptides, 14, 735 (1993)
• K.Furuya et al., Biol. Chem. Hoppe-Seyler, 374, 1065 (1993)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.