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Product Name
CRTAP Polyclonal Antibody
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Description
Polyclonal antibody to CRTAP
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CRTAP antibody; CASP antibody; LEPREL3 antibody; OI7 antibody; P3H5 antibody; cartilage-associated protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human CRTAP (NP_006362.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CRTAP Polyclonal Antibody
Western blot analysis of extracts of SW480 cells, using CRTAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - CRTAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using CRTAP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CRTAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CRTAP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CRTAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using CRTAP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CRTAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using CRTAP antibody at dilution of 1:100 (40x lens).
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Background
Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"