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Product Name
CRHR1 Polyclonal Antibody
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Description
Polyclonal antibody to CRHR1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CRHR1 antibody; CRF-R antibody; CRF-R-1 antibody; CRF-R1 antibody; CRF1 antibody; CRFR-1 antibody; CRFR1 antibody; CRH-R-1 antibody; CRH-R1 antibody; CRHR antibody; CRHR1L antibody; corticotropin-releasing factor receptor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human CRHR1 (NP_001138618.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CRHR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CRHR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
Immunohistochemistry - CRHR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CRHR1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CRHR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CRHR1 antibody at dilution of 1:100 (40x lens).
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Background
G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"