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Product Name
CRH Polyclonal Antibody
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Description
Polyclonal antibody to CRH
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
CRH antibody; CRF antibody; CRH1 antibody; corticoliberin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-194 of human CRH (NP_000747.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CRH Polyclonal Antibody
Western blot analysis of extracts of human brain, using CRH antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"