-
Product Name
CREB3L1,OASIS antibody
- Documents
-
Description
CREB3L1,OASIS Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive IHC detected in human prostate cancer tissue. Positive WB detected in A549 cells, COLO 320 cells. Observed molecular weight by Western-blot: 57 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CREB3L1 antibody; OASIS antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CREB3L1,OASIS recombinant protein (Accession Number: NM_052854). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:109547(CREB3L1,OASIS antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109547(CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:109547(CREB3L1,OASIS Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell.
-
References
- Vellanki RN, Zhang L, Volchuk A. OASIS/CREB3L1 is induced by endoplasmic reticulum stress in human glioma cell lines and contributes to the unfolded protein response, extracellular matrix production and cell migration. PloS one. 8(1):e54060. 2013.
- Rose M, Schubert C, Dierichs L. OASIS/CREB3L1 is epigenetically silenced in human bladder cancer facilitating tumor cell spreading and migration in vitro. Epigenetics. 9(12):1626-40. 2014.
- Ward AK, Mellor P, Smith SE. Epigenetic silencing of CREB3L1 by DNA methylation is associated with high-grade metastatic breast cancers with poor prognosis and is prevalent in triple negative breast cancers. Breast cancer research : BCR. 18(1):12. 2016.
- Vellanki RN, Zhang L, Guney MA, Rocheleau JV, Gannon M, Volchuk A. OASIS/CREB3L1 induces expression of genes involved in extracellular matrix production but not classical endoplasmic reticulum stress response genes in pancreatic beta-cells. Endocrinology. 151(9):4146-57. 2010.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"