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CREB3 antibody

Cat.#: 109546

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Product Information

  • Product Name
    CREB3 antibody
  • Documents
  • Description
    CREB3 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells, Jurkat cells. Observed molecular weight by Western-blot: 40-64kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CREB 3 antibody; CREB3 antibody; LUMAN antibody; LZIP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CREB3 recombinant protein (Accession Number: NM_006368). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109546(CREB3 antibody) at dilution of 1:300

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109546(CREB3 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109546(CREB3 antibody) at dilution of 1:100 (under 25x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109546(CREB3 antibody) at dilution of 1:100 (under 25x lens)

    IP Result of anti-CREB3 (IP:Catalog No:109546, 4ug; Detection:Catalog No:109546 1:500) with PC-3 cells lysate 1800ug.

    IP Result of anti-CREB3 (IP:Catalog No:109546, 4ug; Detection:Catalog No:109546 1:500) with PC-3 cells lysate 1800ug.

  • Background
    CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd.
  • References
    • DenBoer LM, Iyer A, McCluggage AR, Li Y, Martyn AC, Lu R. JAB1/CSN5 inhibits the activity of Luman/CREB3 by promoting its degradation. Biochimica et biophysica acta. 1829(9):921-9. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"