CREB1 antibody

CREB1 antibody

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CREB1 Rabbit Polyclonal antibody. Positive FC detected in HEK-293 cells. Positive IHC detected in human thyroid tissue, human prostate cancer tissue. Positive IF detected in HEK-293 cells. Positive WB detected in multi-cells/tissue, COS-7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, mouse lung tissue, NIH/3T3 cells, rat brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 43-46 kDa

ELISA, IP, WB, IF, IHC, FC

Human, Mouse, Rat, Monkey; other species not tested.

CREB antibody; CREB 1 antibody; CREB1 antibody

Rabbit IgG

This antibody was obtained by immunization of CREB1 recombinant protein (Accession Number: NM_134442). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

• Huang SH, Wang J, Sui WH. BDNF-dependent recycling facilitates TrkB translocation to postsynaptic density during LTP via a Rab11-dependent pathway. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(21):9214-30. 2013.