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Product Name
Creatine kinase B type-Specific antibody
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Description
Creatine kinase B type-Specific Rabbit Polyclonal antibody. Positive IHC detected in human gliomas. Positive WB detected in HEK-293 cells, mouse colon tissue, Y79 cells. Observed molecular weight by Western-blot: 43kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B CK antibody; CKB antibody; CKBB antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; creatine kinase antibody; brain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001823). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109544(CKB-Specific antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109544(CKB-Specific antibody) at dilution of 1:100 (under 40x lens)
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Background
CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
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