-
Product Name
Creatine Kinase antibody
- Documents
-
Description
Creatine Kinase Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 43kd
-
Tested applications
ELISA, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CKMM antibody; Creatine Kinase antibody; Creatine Kinase MM antibody; Creatine kinase M chain antibody; Creatine kinase M type antibody; creatine kinase antibody; muscle antibody; M CK antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Creatine Kinase recombinant protein (Accession Number: NM_001824). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
-
Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:109541(CKM antibody) at dilution of 1:1000
-
Background
Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"