Creatine Kinase antibody

Cat.#: 109541

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Product Information

  • Product Name
    Creatine Kinase antibody
  • Documents
  • Description
    Creatine Kinase Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 43kd
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CKMM antibody; Creatine Kinase antibody; Creatine Kinase MM antibody; Creatine kinase M chain antibody; Creatine kinase M type antibody; creatine kinase antibody; muscle antibody; M CK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Creatine Kinase recombinant protein (Accession Number: NM_001824). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

  • Validations

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:109541(CKM antibody) at dilution of 1:1000

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:109541(CKM antibody) at dilution of 1:1000

  • Background
    Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"