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Product Name
CRAT Polyclonal Antibody
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Description
Polyclonal antibody to CRAT
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CRAT antibody; CAT antibody; CAT1 antibody; carnitine O-acetyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 357-626 of human CRAT (NP_000746.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - CRAT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CRAT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - CRAT Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CRAT antibody . Blue: DAPI for nuclear staining.
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Background
Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. May be specific for short chain fatty acids.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"