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Product Name
CPSF7 antibody
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Description
CPSF7 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human skin tissue. Positive IF detected in HeLa cells. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, mouse uterus tissue. Observed molecular weight by Western-blot: 52 kDa or 56 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CPSF 59 kDa subunit antibody; CPSF7 antibody; FLJ12529 antibody; FLJ39024 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001136040). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109529(CPSF7 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:109529(CPSF7 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109529(CPSF7 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"