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Product Name
CPSF3L Polyclonal Antibody
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Description
Polyclonal antibody to CPSF3L
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
INTS11 antibody; CPSF3L antibody; CPSF73L antibody; INT11 antibody; RC-68 antibody; RC68 antibody; integrator complex subunit 11 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human CPSF3L (NP_060341.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - CPSF3L Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CPSF3L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CPSF3L Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CPSF3L antibody . Blue: DAPI for nuclear staining.
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Background
Catalytic component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"