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Product Name
CPEB4 antibody
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Description
CPEB4 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive WB detected in SH-SY5Y cells, A375 cells, HeLa cells. Observed molecular weight by Western-blot: 80 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CPE binding protein 4 antibody; CPE BP4 antibody; CPEB4 antibody; hCPEB 4 antibody; KIAA1673 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_030627). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:109507(CPEB4 Antibody) at dilution of 1:600
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109507(CPEB4 Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Acetone) fixed SH-SY5Y cells using Catalog No:109507(CPEB4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CPEB4 also named as CPE binding protein 4 or hCPEB 4 is a 729 amino acid protein, which contains two RRM domains and belongs to the RRM CPEB family. CPEB4 involves in some forms of synaptic plasticity and regulate translation by binding to regulatory motifs of defined mRNA targets. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases, generating the need of two sequential CPEB activities to sustain cytoplasmic polyadenylation during all the meiotic phases.
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