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Product Name
COX5A Polyclonal Antibody
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Description
Polyclonal antibody to COX5A
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
COX5A antibody; COX antibody; COX-VA antibody; VA antibody; cytochrome c oxidase subunit 5A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:10 - 1:100 -
Validations
Western blot - COX5A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using COX5A Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - COX5A Polyclonal Antibody
Western blot analysis of extracts of various cells, using COX5A antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunofluorescence - COX5A Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using COX5A antibody . Blue: DAPI for nuclear staining.
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Background
This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"