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Product Name
Connexin-32 antibody
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Description
Connexin-32 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, A375 cells, human liver tissue, mouse skin tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CMTX antibody; CMTX1 antibody; Connexin-32 antibody; CX32 antibody; Gap junction beta 1 protein antibody; GJB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Connexin-32 recombinant protein (Accession Number: XM_017029408). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109452(GJB1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109452(GJB1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109452(GJB1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-Cx32 (IP:Catalog No:109452, 4ug; Detection:Catalog No:109452 1:300) with mouse brain tissue lysate 4000ug.
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Background
Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. (PMID: 8266101; 8613761; 8608591; 19265674)
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References
- Nakase T, Maeda T, Yoshida Y, Nagata K. Ischemia alters the expression of connexins in the aged human brain. Journal of biomedicine & biotechnology. 2009:147946. 2009.
- Yi S, Chen Y, Wen L, Yang L, Cui G. Expression of connexin 32 and connexin 43 in acute myeloid leukemia and their roles in proliferation. Oncology letters. 4(5):1003-1007. 2012.
- Zhong WQ, Chen G, Zhang W. Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features. Histopathology. 66(6):798-807. 2015.
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