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Product Name
Collagen Type IV antibody
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Description
Collagen Type IV Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in rat aorta tissue. Observed molecular weight by Western-blot: 161 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASLN antibody; ATS antibody; CA54 antibody; COL4A5 antibody; Collagen alpha 5(IV) chain antibody; Collagen Type IV antibody; collagen antibody; type IV antibody; alpha 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000495). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
WB result of anti-COL4A5 (Catalog No:109382) with rat aorta.
Immunofluorescent analysis of HepG2 cells using Catalog No:109382(COL4A5 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
COL4A5 belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X-linked (APSX). Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are the cause of diffuse leiomyomatosis with Alport syndrome (DL-ATS)which also known as esophageal and vulval leiomyomatosis with nephropathy or Alport syndrome and diffuse leiomyomatosis (ATS-DL).
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References
- Xiao Q, Jiang Y, Liu Q. Minor Type IV Collagen α5 Chain Promotes Cancer Progression through Discoidin Domain Receptor-1. PLoS genetics. 11(5):e1005249. 2015.
- Chuang TD, Pearce WJ, Khorram O. miR-29c induction contributes to downregulation of vascular extracellular matrix proteins by glucocorticoids. American journal of physiology. Cell physiology. 309(2):C117-25. 2015.
- Khorram O, Chuang TD, Pearce WJ. Long-term effects of maternal undernutrition on offspring carotid artery remodeling: role of miR-29c. Journal of developmental origins of health and disease. 6(4):342-9. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"