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Product Name
Collagen Type IV antibody
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Description
Collagen Type IV Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in HeLa cells, A431 cells, SGC-7901 cells. Observed molecular weight by Western-blot: 50~55 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
COL4A6 antibody; Collagen alpha 6(IV) chain antibody; Collagen Type IV antibody; collagen antibody; type IV antibody; alpha 6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Collagen Type IV recombinant protein (Accession Number: BC005305). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109381(COL4A6 antibody) at dilution of 1:1000
IP Result of anti-COL4A6 (IP:Catalog No:109381, 4ug; Detection:Catalog No:109381 1:300) with mouse skeletal muscle tissue lysate 3600ug.
Immunofluorescent analysis of HeLa cells using Catalog No:109381(COL4A6 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
COL4A6 represents collagen, type IV, alpha 6
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