Collagen Type IV antibody

Cat.#: 109381

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Product Information

  • Product Name
    Collagen Type IV antibody
  • Documents
  • Description
    Collagen Type IV Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in HeLa cells, A431 cells, SGC-7901 cells. Observed molecular weight by Western-blot: 50~55 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    COL4A6 antibody; Collagen alpha 6(IV) chain antibody; Collagen Type IV antibody; collagen antibody; type IV antibody; alpha 6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Collagen Type IV recombinant protein (Accession Number: BC005305). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109381(COL4A6 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109381(COL4A6 antibody) at dilution of 1:1000

    IP Result of anti-COL4A6 (IP:Catalog No:109381, 4ug; Detection:Catalog No:109381 1:300) with mouse skeletal muscle tissue lysate 3600ug.

    IP Result of anti-COL4A6 (IP:Catalog No:109381, 4ug; Detection:Catalog No:109381 1:300) with mouse skeletal muscle tissue lysate 3600ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:109381(COL4A6 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:109381(COL4A6 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    COL4A6 represents collagen, type IV, alpha 6

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"