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Product Name
Human CLPS / Colipase (His Tag) recombinant protein
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Description
Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.
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Protein name
Colipase
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Protein short names
2200003J09RIK; PANCREATIC COLIPASE PREPROPROTEIN; COLIPASE; RP23-480B9.2
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Uniprot ID
P04118
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Gene Name
CLPS
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human CLPS consists of 105 amino acids and predicts a molecular mass of 11.5 kDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human CLPS-His at 10μg/mL(100μL/well) can bind biotinylated human PNLIP-His (Cat:501007).
The EC50 of biotinylated human PNLIP-His (Cat:501007) is 0.57-1.33μg/mL. -
Validations
Human CLPS / Colipase Protein (His Tag) SDS-PAGE
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