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Product Name
COL4A3BP antibody
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Description
COL4A3BP Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HEK-293 cells, human pancreas tissue, mouse kidney tissue, mouse pancreas tissue, rat brain tissue. Observed molecular weight by Western-blot: 80-89 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ceramide transfer protein antibody; CERT antibody; CERTL antibody; COL4A3BP antibody; FLJ20597 antibody; GPBP antibody; hCERT antibody; STARD11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COL4A3BP recombinant protein (Accession Number: NM_031361). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109373(COL4A3BP antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:109373(COL4A3BP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:109373(COL4A3BP antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-COL4A3BP (IP:Catalog No:109373, 4ug; Detection:Catalog No:109373 1:300) with mouse brain tissue lysate 3680ug.
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Background
COL4A3BP(Collagen type IV alpha-3-binding protein) is also named as CERT, STARD11. CERT extracts ceramide from the ER and carries it to the Golgi apparatus in a non-vesicular manner and that a particularly efficient cycle of CERT movement for trafficking of ceramide may proceed at membrane contact sites between the ER and the Golgi apparatus(PMID:17314061). This protein has 3 isoforms produced by alternative splicing.
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