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Product Name
COL1A1 Polyclonal Antibody
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Description
Polyclonal antibody to COL1A1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
COL1A1 antibody; EDSC antibody; OI1 antibody; OI2 antibody; OI3 antibody; OI4 antibody; collagen alpha-1 chain antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human COL1A1 (NP_000079.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - COL1A1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using COL1A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunohistochemistry - COL1A1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using COL1A1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - COL1A1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using COL1A1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - COL1A1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using COL1A1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - COL1A1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - COL1A1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - COL1A1 Polyclonal Antibody
Immunofluorescence analysis of rat ovary using COL1A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - COL1A1 Polyclonal Antibody
Immunofluorescence analysis of human breast cancer using COL1A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - COL1A1 Polyclonal Antibody
Immunofluorescence analysis of human breast cancer using COL1A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Type I collagen is a member of group I collagen (fibrillar forming collagen).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"