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Product Name
CNOT8 Polyclonal Antibody
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Description
Polyclonal antibody to CNOT8
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CNOT8 antibody; CAF1 antibody; CALIF antibody; Caf1b antibody; POP2 antibody; hCAF1 antibody; CCR4-NOT transcription complex subunit 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CNOT8 (NP_004770.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CNOT8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CNOT8 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CNOT8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using CNOT8 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CNOT8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using CNOT8 antibody at dilution of 1:100 (40x lens).
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Background
Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"