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Product Name
CNOT8 antibody
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Description
CNOT8 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human prostate cancer tissue, human kidney tissue. Positive WB detected in Jurkat cells, HeLa cells, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAF1 antibody; CAF1 like protein antibody; CAF2 antibody; CALIF antibody; CALIFp antibody; CCR4 assOCiated factor 8 antibody; CNOT8 antibody; hCAF1 antibody; POP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNOT8 recombinant protein (Accession Number: XM_047417881). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109354(CNOT8 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109354(CNOT8 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CNOT8 (IP:Catalog No:109354, 4ug; Detection:Catalog No:109354 1:600) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109354(CNOT8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.
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References
- Samanfar B, Omidi K, Hooshyar M. Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae. Molecular bioSystems. 9(6):1351-9. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"