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Product Name
CNOT7 antibody
- Documents
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Description
CNOT7 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 33-35 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BTG1 binding factor 1 antibody; CAF 1 antibody; CAF1 antibody; CCR4 assOCiated factor 1 antibody; CNOT7 antibody; hCAF 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNOT7 recombinant protein (Accession Number: XM_047421731). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109353(CNOT7 antibody) at dilution of 1:500
IP Result of anti-CNOT7 (IP:Catalog No:109353, 4ug; Detection:Catalog No:109353 1:300) with mouse testis tissue lysate 4800ug.
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Background
Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved in global gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].
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References
- Sun G, Liu Q, Cheng Y, Hu W. B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis. International journal of molecular medicine. 35(3):777-83. 2015.
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