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Product Name
CNOT3 antibody
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Description
CNOT3 Rabbit Polyclonal antibody. Positive WB detected in Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue. Positive IP detected in HeLa cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CCR4 assOCiated factor 3 antibody; CNOT3 antibody; KIAA0691 antibody; LENG2 antibody; NOT3 antibody; NOT3H antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNOT3 recombinant protein (Accession Number: NM_014516). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109434(CNOT3 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using CNOT3 antibody Catalog No:109434 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CNOT3 (IP:Catalog No:109434, 4ug; Detection:Catalog No:109434 1:1000) with HeLa cells lysate 3200ug.
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Background
The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].
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References
- Neely GG, Kuba K, Cammarato A. A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Cell. 141(1):142-53. 2010.
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