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CNOT2 antibody

Cat.#: 109433

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Product Information

  • Product Name
    CNOT2 antibody
  • Documents
  • Description
    CNOT2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 60 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CCR4 assOCiated factor 2 antibody; CDC36 antibody; CNOT2 antibody; FLJ26456 antibody; HSPC131 antibody; MSTP046 antibody; NOT2 antibody; NOT2H antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CNOT2 recombinant protein (Accession Number: XM_011538399). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IF: 1:50-1:500

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109433(CNOT2 Antibody) at dilution of 1:300

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109433(CNOT2 Antibody) at dilution of 1:300

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:109433(CNOT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:109433(CNOT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The Ccr4–Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
  • References
    • Zheng X, Dumitru R, Lackford BL. Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation. Stem cells (Dayton, Ohio). 30(5):910-22. 2012.
    • Kamon M, Katano M, Hiraki-Kamon K. Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells. Stem cells and development. 23(18):2170-9. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"