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Product Name
CNOT10 antibody
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Description
CNOT10 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, human testis tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 82 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CNOT10 antibody; DKFZp434K115 antibody; FLJ12890 antibody; FLJ13165 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNOT10 recombinant protein (Accession Number: NM_001256741). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109432(CNOT10 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109432(CNOT10 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109432(CNOT10 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CNOT10 antibody Catalog No:109432 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CNOT10 (IP:Catalog No:109432, 4ug; Detection:Catalog No:109432 1:300) with mouse brain tissue lysate 5200ug.
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Background
CNOT10, also named as CCR4-NOT transcription complex subunit 10, is a 744 amino acid protein, which belongs to the CNOT10 family. CNOT10 lacalizes in the cytoplasm and nucleus. CNOT10 is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.
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References
- Färber V, Erben E, Sharma S, Stoecklin G, Clayton C. Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs. Nucleic acids research. 41(2):1211-22. 2013.
- Mauxion F, Prève B, Séraphin B. C2ORF29/CNOT11 and CNOT10 form a new module of the CCR4-NOT complex. RNA biology. 10(2):267-76. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"