-
Product Name
CNKSR3 antibody
- Documents
-
Description
CNKSR3 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 62-65 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CNK homolog protein 3 antibody; CNK3 antibody; CNKSR family member 3 antibody; CNKSR3 antibody; Connector enhancer of KSR 3 antibody; FLJ31349 antibody; MAGI1 antibody; Maguin like protein antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CNKSR3 recombinant protein (Accession Number: NM_173515). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109426(CNKSR3 antibody) at dilution of 1:600
IP Result of anti-CNKSR3 (IP:Catalog No:109426, 4ug; Detection:Catalog No:109426 1:500) with mouse brain tissue lysate 3440ug.
-
Background
Connector enhancer of kinase suppressor of ras 3 (CNKSR3) gene encodes CNK homolog protein 3 (CNK3). CNK proteins, originally identified in Drosophila, were described to regulate the RAS/ERK pathway at the level of the RAS protein kinase. CNKSR3, homologue of scaffold proteins involved in MAPK pathway regulation, is a direct target of mineralocorticoid receptor (MR). CNK3 coordinates the assembly of a multiprotein epithelial sodium channel (ENaC) -regulatory complex, and hence plays a central role in sodium homeostasis.
-
References
- Soundararajan R, Ziera T, Koo E. Scaffold protein connector enhancer of kinase suppressor of Ras isoform 3 (CNK3) coordinates assembly of a multiprotein epithelial sodium channel (ENaC)-regulatory complex. The Journal of biological chemistry. 287(39):33014-25. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"