CMX-8933 peptide

CMX-8933 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KKETLQFR-OH

H-Lys-Lys-Glu-Thr-Leu-Gln-Phe-Arg-OH

8

95.48%

C₄₇H₈₀N₁₄O₁₃

1049.22

146877-90-9

11457457

Synthetic

Powder

CMX-8933 is an 8-amino acid peptide fragment of Ependymin, a goldfish brain glycoprotein. CMX-8933 has the function of activating the AP-1 transcription factor in both mouse neuroblastoma (NB2a) and primary rat brain cortical cell cultures. In NB2a cells, a 13–14-fold increase in AP-1’s DNA binding was obtained with 6–10 mg/ml CMX-8933 after 4 h incubation, and in primary rat brain cortical cultures, a 26.7 ± 5.2-fold increase in binding was observed with as little as 10 ng/ml peptide after 3 h treatment. The activation of AP-1 transcription factor by CMX-8933 is rapid and transient, and it may play a role in transcriptional control, which is characteristic of full-sized neurotrophic factors in neuronal differentiation and regeneration. This suggests a potential role for Ependymin, from which CMX-8933 is derived, in transcriptional control and neuroplasticity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Shashoua VE, Adams D, Boyer-Boiteau A. CMX-8933, a peptide fragment of the glycoprotein ependymin, promotes activation of AP-1 transcription factor in mouse neuroblastoma and rat cortical cell cultures. Neurosci Lett. 2001 Oct 19;312(2):103-7. doi: 10.1016/s0304-3940(01)02119-x. PMID: 11595345.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.