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Product Name
CLUAP1 antibody
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Description
CLUAP1 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells. Positive IP detected in HepG2 cells. Positive IF detected in MDCK cells. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLUAP1 antibody; clusterin assOCiated protein 1 antibody; FLJ13297 antibody; KIAA0643 antibody; Qilin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLUAP1 recombinant protein (Accession Number: NM_015041). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:109409(CLUAP1 Antibody) at dilution of 1:600
IP Result of anti-CLUAP1 (IP:Catalog No:109409, 4ug; Detection:Catalog No:109409 1:600) with HepG2 cells lysate 2400ug.
Immunofluorescent analysis of MDCK cells using Catalog No:109409(CLUAP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).
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