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Product Name
CLTC Polyclonal Antibody
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Description
Polyclonal antibody to CLTC
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CLTC antibody; CHC antibody; CHC17 antibody; CLH-17 antibody; CLTCL2 antibody; Hc antibody; clathrin heavy chain antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1451-1675 of human CLTC (NP_004850.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CLTC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CLTC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - CLTC Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using CLTC antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"