CLOCK antibody

CLOCK antibody

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CLOCK Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells, PC-3 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 150 kDa

ELISA, WB, IP, IF

Human; other species not tested.

bHLHe8 antibody; CLOCK antibody; clOCk homolog (mouse) antibody; hCLOCK antibody; KAT13D antibody; KIAA0334 antibody

Rabbit IgG

This antibody was obtained by immunization of CL℃K recombinant protein (Accession Number: XM_047416433). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Circadian locomoter output cycles protein kaput (CLocK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLocK localizes in the nucleus and cytoplasm. CLocK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLocK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLocK is 95kDa, but we detected a 150 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 9808471).

• Cui M, Zheng M, Sun B, Wang Y, Ye L, Zhang X. A long noncoding RNA perturbs the circadian rhythm of hepatoma cells to facilitate hepatocarcinogenesis. Neoplasia (New York, N.Y.). 17(1):79-88. 2015.