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Product Name
CLN5 Polyclonal Antibody
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Description
Polyclonal antibody to CLN5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CLN5 antibody; NCL antibody; ceroid-lipofuscinosis, neuronal 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 96-407 of human CLN5 (NP_006484.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CLN5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CLN5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CLN5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using CLN5 antibody at dilution of 1:150 (40x lens).
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Background
Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"