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Product Name
CLN3 antibody
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Description
CLN3 Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 45-48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Batten disease protein antibody; BATTENIN antibody; BTS antibody; CLN3 antibody; JNCL antibody; Protein CLN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLN3 recombinant protein (Accession Number: NM_000086). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:109396(CLN3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human brain using Catalog No:109396(CLN3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109396(CLN3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CLN3 antibody Catalog No:109396 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).
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