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Product Name
CLIC3 antibody
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Description
CLIC3 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 27-32 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CLIC3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLIC3 recombinant protein (Accession Number: NM_004669). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109389(CLIC3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109389(CLIC3 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109389(CLIC3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using CLIC3 antibody Catalog No:109389 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.
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References
- Liddelow SA, Dziegielewska KM, Ek CJ. Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach. PloS one. 8(7):e65629. 2013.
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