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Product Name
CLIC1 antibody
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Description
CLIC1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HL-60 cells, HeLa cells. Positive IHC detected in human small intestine tissue, human colon tissue, human kidney tissue, human pancreas tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 27-32 kDa
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Tested applications
ELISA, IF, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Chloride channel ABP antibody; CLIC1 antibody; hRNCC antibody; NCC27 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CLIC1 recombinant protein (Accession Number: NM_001288). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109388(CLIC1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:109388(CLIC1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:109388(CLIC1 Antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CLIC1 antibody Catalog No:109388 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CLIC1 (IP:Catalog No:109388, 4ug; Detection:Catalog No:109388 1:300) with HeLa cells lysate 1200ug.
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Background
CLIC1 represents chloride intracellular channel 1
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