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Product Name
CLDN4 Polyclonal Antibody
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Description
Polyclonal antibody to CLDN4
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
CLDN4 antibody; CPE-R antibody; CPER antibody; CPETR antibody; CPETR1 antibody; WBSCR8 antibody; hCPE-R antibody; claudin-4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CLDN4 (NP_001296.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - CLDN4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CLDN4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - CLDN4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using CLDN4 antibody at dilution of 1:150 (40x lens).
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Background
Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"