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Product Name
CLCN5 Polyclonal Antibody
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Description
Polyclonal antibody to CLCN5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CLCN5 antibody; CLC5 antibody; CLCK2 antibody; ClC-5 antibody; DENTS antibody; NPHL1 antibody; NPHL2 antibody; XLRH antibody; XRN antibody; hCIC-K2 antibody; chloride voltage-gated channel 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-816 of human CLCN5 (NP_001121370.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CLCN5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CLCN5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CLCN5 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CLCN5 antibody . Blue: DAPI for nuclear staining.
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Background
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"