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Product Name
CKLF Polyclonal Antibody
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Description
Polyclonal antibody to CKLF
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
CKLF antibody; C32 antibody; CKLF1 antibody; CKLF2 antibody; CKLF3 antibody; CKLF4 antibody; HSPC224 antibody; UCK-1 antibody; chemokine-like factor antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-67 of human CKLF (NP_057410.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CKLF Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CKLF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
May play an important role in inflammation and regeneration of skeletal muscle. Partly inhibited by interleukin 10.; Isoform 1: has chemotactic response in monocytes, neutrophils and lymphocytes. Binds CCR4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"