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Human CNTF/Ciliary Neurotrophic Factor (His Tag) recombinant protein

Cat.#: 500327

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Product Information

  • Product Name
    Human CNTF/Ciliary Neurotrophic Factor (His Tag) recombinant protein
  • Documents
  • Description
    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • Protein name
    Ciliary neurotrophic factor
  • Protein short names
    AI429687; CNTF; MGC41235; HCNTF
  • Uniprot ID
    P26441
  • Gene Name
    CNTF
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) was expressed, with a polyhistide tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human CNTF consisting of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The apparent molecular mass of the protein is approximately 27-30 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA. Immobilized human His-CNTF (cat:500327) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (Cat:501299). The EC50 of biotinylated rat CNTFR-His (Cat:501299) is 60-140 ng/ml.
    2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 μg/mL.
  • Validations

    Human CNTF Protein (His Tag) SDS-PAGE

    Human CNTF Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"