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Product Name
CIB1 antibody
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Description
CIB1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human testis tissue, rat testis tissue. Positive IP detected in mouse testis tissue. Positive WB detected in human liver tissue, HepG2 cells, rat testis tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Calmyrin antibody; CIB antibody; CIB1 antibody; CIBP antibody; DNA PKcs interacting protein antibody; Kinase interacting protein antibody; KIP antibody; KIP1 antibody; PRKDCIP antibody; SIP2 28 antibody; SNK interacting protein 2 28 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CIB1 recombinant protein (Accession Number: NM_006384). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109311(CIB1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109311(CIB1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-CIB1 (IP:Catalog No:109311, 4ug; Detection:Catalog No:109311 1:300) with mouse testis tissue lysate 4000ug.
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Background
CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.
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References
- Bandyopadhyay C, Valiya-Veettil M, Dutta D, Chakraborty S, Chandran B. CIB1 synergizes with EphrinA2 to regulate Kaposi's sarcoma-associated herpesvirus macropinocytic entry in human microvascular dermal endothelial cells. PLoS pathogens. 10(2):e1003941. 2014.
- Bandyopadhyay C, Veettil MV, Dutta S, Chandran B. p130Cas scaffolds the signalosome to direct adaptor-effector cross talk during Kaposi's sarcoma-associated herpesvirus trafficking in human microvascular dermal endothelial cells. Journal of virology. 88(23):13858-78. 2014.
- Witkiewicz H, Oh P, Schnitzer JE. III. Cellular ultrastructures in situ as key to understanding tumor energy metabolism: biological significance of the Warburg effect. F1000Research. 2:10. 2013.
- Witkiewicz H, Oh P, Schnitzer JE. I. Embryonal vasculature formation recapitulated in transgenic mammary tumor spheroids implanted pseudo-orthotopicly into mouse dorsal skin fold: the organoblasts concept. F1000Research. 2:8. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"