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Product Name
CIAO1 Polyclonal Antibody
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Description
Polyclonal antibody to CIAO1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CIAO1 antibody; CIA1 antibody; WDR39 antibody; cytosolic iron-sulfur assembly component 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human CIAO1 (NP_004795.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CIAO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CIAO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - CIAO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CIAO1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CIAO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using CIAO1 antibody at dilution of 1:100 (40x lens).
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Background
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"