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Product Name
CHST13-Specific antibody
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Description
CHST13-Specific Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human kidney tissue, human hepat℃irrhosis tissue. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C4ST 3 antibody; C4ST3 antibody; CHST13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_152889). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109294(CHST13-Specific antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109294(CHST13-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109294(CHST13-Specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CHST13 antibody Catalog No:109294 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CHST13-Specific (IP:Catalog No:109294, 4ug; Detection:Catalog No:109294 1:500) with mouse liver tissue lysate 4000ug.
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Background
CHST13, also named as C4ST3, belongs to the sulfotransferase 2 family. CHST13 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. CHST13 transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. The antibody is specific to CHST13.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"