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Product Name
CHRNE Polyclonal Antibody
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Description
Polyclonal antibody to CHRNE
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHRNE antibody; ACHRE antibody; CMS1D antibody; CMS1E antibody; CMS2A antibody; CMS4A antibody; CMS4B antibody; CMS4C antibody; FCCMS antibody; SCCMS antibody; acetylcholine receptor subunit epsilon antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of human CHRNE (NP_000071.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:4000
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Validations
Western blot - CHRNE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHRNE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"