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Product Name
CHRNA4 Polyclonal Antibody
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Description
Polyclonal antibody to CHRNA4
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Tested applications
WB
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Species reactivity
Mouse
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Alternative names
CHRNA4 antibody; BFNC antibody; EBN antibody; EBN1 antibody; NACHR antibody; NACHRA4 antibody; NACRA4 antibody; cholinergic receptor nicotinic alpha 4 subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human CHRNA4
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CHRNA4 Polyclonal Antibody
Western blot analysis of extracts of mouse kidney, using CHRNA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"