Cart 2

CHRNA3 antibody

Cat.#: 109279

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    CHRNA3 antibody
  • Documents
  • Description
    CHRNA3 Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, A549 cells, HEK-293 cells, HepG2 cells, mouse brain tissue. Positive IHC detected in human prostate cancer tissue. Positive FC detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CHRNA3 antibody; LNCR2 antibody; NACHRA3 antibody; PAOD2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CHRNA3 recombinant protein (Accession Number: NM_000743). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:109279(CHRNA3 antibody) at dilution of 1:1000

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:109279(CHRNA3 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109279(CHRNA3 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109279(CHRNA3 antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNA3 antibody (Catalog No:109279, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNA3 antibody (Catalog No:109279, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.
  • References
    • Zhao Y, Zhou W, Xue L, Zhang W, Zhan Q. Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC). PloS one. 9(3):e90836. 2014.
    • Perez-Burgos A, Mao YK, Bienenstock J, Kunze WA. The gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse". FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(7):3064-74. 2014.
    • Strunnikova NV, Maminishkis A, Barb JJ. Transcriptome analysis and molecular signature of human retinal pigment epithelium. Human molecular genetics. 19(12):2468-86. 2010.
    • Soeda J, Morgan M, McKee C. Nicotine induces fibrogenic changes in human liver via nicotinic acetylcholine receptors expressed on hepatic stellate cells. Biochemical and biophysical research communications. 417(1):17-22. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"