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Product Name
CHRM1 Polyclonal Antibody
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Description
Polyclonal antibody to CHRM1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHRM1 antibody; HM1 antibody; M1 antibody; M1R antibody; cholinergic receptor muscarinic 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CHRM1 (NP_000729.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CHRM1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - CHRM1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CHRM1 antibody at dilution of 1:100 (40x lens).
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Background
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"